Archives
Nanobody-TurboID Maps the CD38 Surfaceome
2026-09-11
Feng and colleagues introduce nanobody-targeted TurboID, or NBID, to define proteins located near CD38 on living cell surfaces. Integrated proximity labeling, quantitative proteomics, microscopy, and migration assays identify a CD38-associated adhesion network and connect it to tumor–endothelial interactions.
Fluorescein TSA Fluorescence System Kit Guide
2026-09-10
The Fluorescein TSA Fluorescence System Kit turns weak protein and nucleic-acid signals into bright, spatially localized fluorescence for IHC, ICC, and ISH. Its most useful role is validating low-level expression and cellular distribution after advanced neuromodulation experiments such as the HcKCR1-hs seizure study.
Phillygenin in Diabetic Nephropathy: Mechanistic Evidence
2026-09-10
The reference study shows that phillygenin protects against diabetic nephropathy in high-glucose podocytes and db/db mice by suppressing TLR4/MyD88/NF-κB-associated inflammation while supporting PI3K/AKT/GSK3β signaling and reducing apoptosis. Its integrated RNA-seq, molecular, cellular, and animal-model design provides a useful framework for evaluating candidate interventions, while also highlighting the need to distinguish mechanistic pathway effects from general changes in cell viability.
Renalase–PMCA4b/cAMP Control of Aldosterone
2026-09-09
The 2026 reference study identifies renalase as a stimulatory regulator of aldosterone production in NCI-H295R adrenocortical cells and links this effect to PMCA4b-dependent cAMP/PKA signaling rather than classical calcium activation. Its findings provide a mechanistic framework for understanding renalase in aldosterone-producing pathology while highlighting the need for in vivo and human-tissue validation.
Gut Microbial H₂S Lowers GLP-1 in Male Mice
2026-09-09
A 2024 Nature Metabolism study identifies Desulfovibrio-derived hydrogen sulfide as a gut-to-host signal that suppresses GLP-1 production in male mice. By connecting microbial sulfide production with impaired mitochondrial respiration and an unfolded protein response in intestinal L cells, the work provides a mechanistic explanation for how dysbiosis may worsen metabolic disease and evaluates bismuth subsalicylate as an intervention.
IWR-1-endo Workflow for Wnt Pathway Assays
2026-09-08
IWR-1-endo provides reversible, nanomolar-scale control of the Wnt/β-catenin signaling pathway for colorectal cancer, stem-cell, and regeneration assays. This workflow combines careful DMSO handling, pathway-specific controls, quantitative morphology, and orthogonal validation to improve reproducibility.
PGE2, Schwann Cells, and PDAC Perineural Invasion
2026-09-08
Wang and colleagues identify a paracrine PTGES–PGE2 axis that drives Schwann-cell dedifferentiation and promotes perineural invasion in pancreatic ductal adenocarcinoma. By integrating patient-derived transcriptomic analyses with coculture and three-dimensional perturbation models, the study links PGE2-stimulated Schwann cells to LIF- and ADAMTS-1-associated extracellular-matrix and neural remodeling.
Mithramycin A Workflows for Transcription Research
2026-09-07
Mithramycin A turns selective G-C-rich DNA binding into a practical perturbation strategy for transcription, c-myc regulation, and myeloid differentiation experiments. This guide connects established cancer biology applications with a carefully bounded assay concept inspired by the miR-24-3p/Sp1/PI3K study in doxorubicin-induced cardiac injury.
Tetraethylammonium Chloride: Assay Logic
2026-09-07
Tetraethylammonium chloride is more than a potassium-channel blocker: it is a tool for separating pore-level ion conduction from tissue-level physiology. This guide combines TEAC mechanism, assay design, product handling, and cross-domain interpretation for reproducible research.
Phalloidin B7678: F-Actin Workflow Guide
2026-09-05
Phalloidin (B7678) is a high-affinity F-actin stabilizer for visualizing filament organization in fixed, permeabilized cells, tissue sections, and cell-free assays. It is not appropriate for live-cell imaging or experiments that require reversible actin binding and native cytoskeletal dynamics.
Live-Dead Bacterial Staining Kit: Lab Guide
2026-09-04
Learn how the Live-Dead Bacterial Staining Kit, SKU K2239, supports membrane-based bacterial viability assessment in cytotoxicity and nanomaterial studies. This scenario-driven guide covers assay design, protocol controls, interpretation, storage, and practical vendor selection.
Dorsomorphin (Compound C) AMPK Study Workflow
2026-09-04
Use Dorsomorphin (Compound C) to test whether AMPK-ACC signaling controls ferroptosis, autophagy, metabolism, or differentiation in cell and animal models. This workflow combines rapid pharmacological inhibition with phospho-signaling, lipid-peroxidation, viability, and genetic controls for more interpretable results.
HPF: Selective hROS Detection in Cells
2026-09-03
HPF, or hydroxyphenyl fluorescein, is a cell-permeable fluorescent probe for highly reactive oxygen species detection. Its oxidation-dependent conversion to fluorescein supports selective intracellular oxidative stress visualization, while defined storage and optical parameters improve assay reproducibility.
Sulfisomidine: Reliable Assay Workflows
2026-09-03
A scenario-driven guide to using Sulfisomidine (SKU BA1099) in viability, enzyme, antibacterial, and transformation studies. It emphasizes solvent compatibility, controls, concentration interpretation, and evidence-based comparison of laboratory supply options.
EZ Cap Cy5 Firefly Luciferase mRNA Guide
2026-09-02
EZ Cap Cy5 Firefly Luciferase mRNA combines a Cap1-capped, 5-moUTP modified mRNA reporter with covalent Cy5 labeling. The design supports parallel measurement of intracellular RNA handling and luciferase expression, while the product specifications define a 1,921-nucleotide transcript supplied at 1 mg/mL in sodium citrate buffer.
708 records 8/48 page Previous Next First page 上5页 678910 下5页 Last page